Peptide Calculator
Three inputs. One answer. Enter your vial size, water volume, and target dose, and get the exact syringe units to draw. No mental math, no guessing.
A free peptide dosing calculator that handles every common research peptide: tirzepatide, semaglutide, retatrutide, BPC-157, ipamorelin, GHK-Cu, and the rest. Below the calculator: a complete peptide reconstitution chart, worked examples per peptide, units conversions, and storage guidance.
What is the total volume of your syringe?
How many milligrams are in your peptide vial?
How much bacteriostatic water are you adding?
How much peptide do you want per dose?
Select your vial size, water amount, and dose above to see your result.
How to reconstitute peptides in four steps
Peptides arrive as freeze-dried powder. To reconstitute peptides for injection, you mix them with bacteriostatic water and let the powder fully dissolve. Proper technique keeps the peptide intact and your doses accurate. The peptide mixing calculator above handles the math; the four steps below handle the procedure.
Clean setup
Sterile gloves, clean surface. Have your peptide vial, BAC water, insulin syringe, and alcohol swabs ready before you start.
Warm to room temperature
If either vial was refrigerated or frozen, let it sit at room temperature (20-25 degrees C) first. Cold liquid causes foaming and poor dissolution.
Add water slowly
Swab both vial tops with alcohol. Draw your BAC water and inject it against the inner glass wall at a 45-degree angle. Do not spray it onto the powder directly.
Swirl, never shake
Gently roll or swirl the vial until the solution is completely clear. Aggressive shaking damages peptide bonds. If it stays cloudy, give it more time.
Unsure how much water to use?
Water volume controls concentration. Less water = fewer units per dose (harder to measure small amounts). More water = more units per dose (easier precision). Try different amounts in the calculator above to see how it changes your syringe draw.
Storage after mixing
Once reconstituted, temperature is everything. The peptide is now in solution and degrades faster than powder. Two options depending on your timeline.
Refrigerated (3-4 weeks)
Keep at 2-8 degrees C in the main compartment of your fridge. Avoid the door shelf where temperature swings on every open. Store upright, stopper facing up.
Frozen (3-4 months)
Split into single-use aliquots and freeze at -20 degrees C. Each freeze-thaw cycle degrades the peptide, so only thaw what you intend to use. Do not refreeze.
Powder is more forgiving. Unreconstituted peptide can sit at room temperature for weeks or stay frozen for years. Only mix what you will actually use in the next few weeks.
The math behind every result
The calculator above does this automatically, but here is the three-step formula so you can verify any result by hand. Worked example: 5 mg vial, 2 ml BAC water, 250 mcg dose.
Concentration
Vial size divided by water volume. This tells you how much peptide is in each ml of solution.
Injection volume
Your dose (converted to mg) divided by the concentration. This is the actual liquid volume you inject.
Syringe units
Injection volume times 100. Every insulin syringe marks 100 units per ml, so this gives you the exact tick mark.
Common setups by peptide
Typical reconstitution configurations for popular peptides. Expand any entry to see the math, then load those values into the calculator.
5 mg vial · 2 ml BAC water · 250 mcg dose · 1-2x daily
One of the most widely used repair peptides. A 5 mg vial with 2 ml water gives you a 2.5 mg/ml solution. At 250 mcg per injection, that is 10 units on the syringe and 20 total doses from a single vial.
Peptide reconstitution chart
Pre-calculated syringe units for the most common vial and water combinations. Functions as a peptide dosing chart for any peptide where the vial size, water volume, and target dose match a row below. All values assume a 100-unit (1 ml) insulin syringe. A dash means the dose exceeds syringe capacity at that concentration.
How to use: Find your vial size and water volume on the left, then read across to your dose column. The number is the unit mark on your syringe.
Example: 5 mg vial + 2 ml water + 250 mcg dose = 10 units.
| Vial | BAC Water | Conc. | 250 mcg | 500 mcg | 1 mg | 2.5 mg | 5 mg |
|---|---|---|---|---|---|---|---|
| 2 mg | 1 ml | 2 mg/ml | 12.5 | 25 | 50 | — | — |
| 2 mg | 2 ml | 1 mg/ml | 25 | 50 | 100 | — | — |
| 5 mg | 1 ml | 5 mg/ml | 5 | 10 | 20 | 50 | 100 |
| 5 mg | 2 ml | 2.5 mg/ml | 10 | 20 | 40 | 100 | — |
| 5 mg | 3 ml | 1.67 mg/ml | 15 | 30 | 60 | — | — |
| 10 mg | 1 ml | 10 mg/ml | 2.5 | 5 | 10 | 25 | 50 |
| 10 mg | 2 ml | 5 mg/ml | 5 | 10 | 20 | 50 | 100 |
| 10 mg | 3 ml | 3.33 mg/ml | 7.5 | 15 | 30 | 75 | — |
| 15 mg | 2 ml | 7.5 mg/ml | 3.3 | 6.7 | 13.3 | 33.3 | 66.7 |
| 15 mg | 3 ml | 5 mg/ml | 5 | 10 | 20 | 50 | 100 |
Units and conversions
Peptide dosing involves five different units. Most confusion comes from mixing up weight (mg, mcg) with volume (ml, units). Here is what each one measures and when you encounter it.
| Unit | Full Name | Measures | Used For |
|---|---|---|---|
| mg | Milligram | Weight | How much peptide is in a vial |
| mcg | Microgram | Weight | How much peptide per injection |
| ml | Milliliter | Volume | BAC water volume and injection volume |
| IU | International Unit | Biological activity | Dosing for HGH and certain hormones |
| Units | Syringe Units | Volume (markings) | The markings on insulin syringes |
Key Conversion
1 mg = 1,000 mcg
A 5 mg vial contains 5,000 mcg total
Syringe Unit to Volume
1 unit = 0.01 ml
30-unit = 0.3 ml, 50-unit = 0.5 ml, 100-unit = 1.0 ml
Peptide dosing at a glance
Typical vial sizes, water volumes, and dose ranges for popular peptides. These are general starting points. Your protocol may differ based on your health profile and goals.
| Peptide | Category | Vial Size | Typical Water | Common Dose |
|---|---|---|---|---|
| BPC-157 | Tissue Repair | 5 mg | 1-2 ml | 250-500 mcg |
| Semaglutide | Weight Loss | 5 mg, 10 mg | 2-2.5 ml | 0.25-2.4 mg |
| Tirzepatide | Weight Loss | 5 mg, 10 mg, 15 mg | 2-3 ml | 2.5-15 mg |
| TB-500 | Tissue Repair | 5 mg, 10 mg | 1-2 ml | 2-5 mg |
| CJC-1295 / Ipamorelin | Growth Hormone | 5 mg blend | 2.5 ml | 300 mcg |
| Sermorelin | Growth Hormone | 5 mg, 9 mg, 15 mg | 2-3 ml | 200-500 mcg |
| AOD-9604 | Weight Loss | 5 mg | 2 ml | 300 mcg |
| PT-141 | Sexual Wellness | 10 mg | 2 ml | 1-2 mg |
Common questions
Everything else about reconstitution, dosing math, and syringe selection.
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